▶Subject: Single-cell and single-molecule transcriptome profiling with RNA modifications
▶Speaker: Kyung Lock Kim, Ph.D.
( Department of Pathology and Center for Cancer Research, Massachusetts General Hospital and Harvard Medical School)
▶Date: 11:00AM/Jan. 31(Thur.)/2019
▶Place: Conference room(#179), Postech Biotech Center
An extensive repertoire of epigenetic modifications is known to influence transcriptional and post-transcriptional steps in gene expression by controlling the versatile coding, structural and catalytical functions. Although diverse bulk sequencing techniques with epigenetic modifications are developed, in-depth study of single-cell epigenetic sequencing (DNA, RNA and histone modifications) has been impeded by a lack of robust analytical methods. Here we present the novel single-cell sequencing method to profile transcriptome with RNA modifications. Using >60k nanowell technique for single-cell isolation and high-throughput single-molecule imaging, we reveal that individual cells exhibit different level of each epigenetic mark in single-cell transcriptome. In addition, we demonstrate the single-molecule sequencing with combinatorial epigenetic marks in individual cells. Finally, we identify cell subpopulations defined by differences in epi-transcriptomic states. Our approach has the potential to uncover combinatorial modifications states on single molecules, and to investigate their variability across single cells and conditions.
▶Inquiry: : Prof. Sung Ho Ryu (279-2292)
* This seminar will be given in English.
Please refrain from taking photos during seminars. *